Ion trap collision-induced dissociation of locked nucleic acids
نویسندگان
چکیده
منابع مشابه
Locked Nucleic Acids (LNAs)
With few exceptions, experimental success in molecular biology is dependent upon specific, discriminating, and persistent hybridization events involving synthetic oligonucleotides and their complementary target sequences. While unmodified oligodeoxynucleotides will routinely form desired DNA:DNA and DNA:RNA duplexes, synthesis of various modifications that confer enhanced high-affinity recognit...
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A method of fragmenting ions over a wide range of m/z values while balancing energy deposition into the precursor ion and available product ion mass range is demonstrated. In the method, which we refer to as "multigenerational collision-induced dissociation", the radiofrequency (rf) amplitude is first increased to bring the lowest m/z of the precursor ion of interest to just below the boundary ...
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The fragmentation of natural peptides using dynamic collision-induced dissociation (DCID), a novel fragmentation method for quadrupole ion traps, is demonstrated. Using leucine enkephalin as a diagnostic molecule, the fragmentation efficiencies and energetics of DCID are compared with other methods of collisional activation in ion traps such as conventional on-resonance excitation and high-ampl...
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Transfer RNA is a class of highly modified and structured non-coding RNA molecules generally comprised of 74-95 nucleotides. In this study, tandem mass spectrometry of intact multiply charged tRNA anions of roughly 25 kDa in mass has been demonstrated using a quadrupole/time-of-flight tandem mass spectrometer adapted for ion/ion reaction studies. The sample proved to be a mixture of tRNA molecu...
متن کاملGas-phase separations of protein and peptide ion fragments generated by collision-induced dissociation in an ion trap.
Ion mobility/time-of-flight mass spectrometry techniques have been used to examine distributions of fragment ions generated by collision-induced dissociation (CID) in a quadrupole ion trap. The mobility-based separation step prior to mass-to-charge (m/z) analysis reduces spectral congestion and provides information that complements m/z-based assignments of peaks. The approach is demonstrated by...
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ژورنال
عنوان ژورنال: Journal of the American Society for Mass Spectrometry
سال: 2010
ISSN: 1044-0305,1879-1123
DOI: 10.1016/j.jasms.2009.09.020